Recombinant Human Erythropoeitin

Recombinant human erythropoeitin: efficacy and safety considerations for maximizing blood conservation in cardiac surgery.

B LOOD products transfused to patients undergoing cardiac surgery consume approximately 15% of the banked blood supply in the United States. Cardiac surgical patients known to be at particular risk for receiving allogeneic blood transfusions in the perioperative period include patients with preoperative anemia and patients undergoing surgeries other than primary coronary artery bypass grafting ...

Corticosteroids and erythropoeitin-receptor agonists.

Cloning, Expression, Purification and CD Analysis of Recombinant Human Betatrophin

Betatrophin is a member of the angiopoietin-like (ANGPTL) family that has been implicated in both triglyceride and glucose metabolism. The physiological functions and molecular targets of this protein remain largely unknown; hence, a purified available protein would aid study of the exact role of betatrophin in lipid or glucose metabolism. In this study, we cloned the full-length cDNA of betatr...

Production of Recombinant Adenovirus Containing Human Interlukin-4 Gene

Objective(s) Recombinant adenoviruses are currently used for a variety of purposes, including in vitro gene transfer, in vivo vaccination, and gene therapy. Ability to infect many cell types, high efficiency in gene transfer, entering both dividing and non dividing cells, and growing to high titers make this virus a good choice for using in various experiments. In the present experiment, a reco...

Recombinant human interferon-y

Recombinant human interferon-y (Hu-IFN-y) produced by Chinese-hamster ovary (CHO) cells was analysed by immunoprecipitation and SDS/PAGE. Up to twelve molecular-mass variants were secreted by this cell line. Three variants were recovered after enzymic removal of all N-linked oligosaccharides or when glycosylation was inhibited by tunicamycin. The presence of three polypeptide forms rather than ...